Analysis of bluetongue serotype 3 spread in Tunisia and discovery of a novel strain related to the bluetongue virus isolated from a commercial sheep pox vaccine.
Identifieur interne : 000434 ( Main/Exploration ); précédent : 000433; suivant : 000435Analysis of bluetongue serotype 3 spread in Tunisia and discovery of a novel strain related to the bluetongue virus isolated from a commercial sheep pox vaccine.
Auteurs : Alessio Lorusso [Italie] ; Soufien Sghaier [Tunisie] ; Marco Di Domenico [Italie] ; Mohamed Elias Barbria [Tunisie] ; Guendalina Zaccaria [Italie] ; Aida Megdich [Tunisie] ; Ottavio Portanti [Italie] ; Imed Ben Seliman [Tunisie] ; Massimo Spedicato [Italie] ; Federica Pizzurro [Italie] ; Irene Carmine [Italie] ; Liana Teodori [Italie] ; Mejdi Mahjoub [Tunisie] ; Iolanda Mangone [Italie] ; Alessandra Leone [Italie] ; Salah Hammami [Tunisie] ; Maurilia Marcacci [Italie] ; Giovanni Savini [Italie]Source :
- Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases [ 1567-7257 ] ; 2018.
Descripteurs français
- KwdFr :
- ARN viral (génétique), ARN viral (sang), Animaux (MeSH), Femelle (MeSH), Fièvre catarrhale du mouton (virologie), Infections à Poxviridae (prévention et contrôle), Infections à Poxviridae (virologie), Maladies des ovins (prévention et contrôle), Maladies des ovins (virologie), Ovis (virologie), Sérogroupe (MeSH), Tunisie (MeSH), Vaccins antiviraux (génétique), Virus de la langue bleue (classification), Virus de la langue bleue (génétique).
- MESH :
- génétique : ARN viral, Vaccins antiviraux, Virus de la langue bleue.
- prévention et contrôle : Infections à Poxviridae, Maladies des ovins.
- sang : ARN viral.
- virologie : Fièvre catarrhale du mouton, Infections à Poxviridae, Maladies des ovins, Ovis.
- Animaux, Femelle, Sérogroupe, Tunisie.
- Wicri :
- geographic : Tunisie.
English descriptors
- KwdEn :
- Animals (MeSH), Bluetongue (virology), Bluetongue virus (classification), Bluetongue virus (genetics), Female (MeSH), Poxviridae Infections (prevention & control), Poxviridae Infections (virology), RNA, Viral (blood), RNA, Viral (genetics), Serogroup (MeSH), Sheep (virology), Sheep Diseases (prevention & control), Sheep Diseases (virology), Tunisia (MeSH), Viral Vaccines (genetics).
- MESH :
- chemical , blood : RNA, Viral.
- chemical , genetics : Viral Vaccines.
- geographic : Tunisia.
- classification : Bluetongue virus.
- genetics : Bluetongue virus, RNA, Viral.
- prevention & control : Poxviridae Infections, Sheep Diseases.
- virology : Bluetongue, Poxviridae Infections, Sheep, Sheep Diseases.
- Animals, Female, Serogroup.
Abstract
Bluetongue (BT), is one of the OIE-listed major diseases of ruminants. Following the official report of BT virus serotype 3 (BTV-3) in a sheep in Cap Bon (Tunisia), blood and serum samples of ruminants were collected from some areas of Tunisia to further investigate the presence of this virus in the country. A quantitative real time RT-PCR has been first developed for the detection and quantitation of BTV-3 RNA from field specimens. Out of 62 collected blood samples, 23 were shown to be positive for BTV-3 RNA. Isolation on cell cultures was also possible from six samples. Genome sequencing revealed the circulation of two unrelated western strains of BTV-3, one circulating in Cap Bon and neighboring areas, and the other circulating nearby the border with Libya. The presence of a putative novel BTV serotype (BTV-Y TUN2017) in sheep introduced from Libya to Tunisia, genomically related to the BTV strain contaminating a commercially-available sheep pox vaccine and to BTV-26, has been also demonstrated. This finding highlights the pressing need for a prompt production and release of a novel inactivated BTV-3 vaccine to be used in case of emergence or proactively in the areas of Southern Europe at major risk of BTV introduction. The assessment of a novel vaccine will certainly exalt the role and importance of surveillance activities and collaboration with Northern African countries.
DOI: 10.1016/j.meegid.2018.01.025
PubMed: 29386141
Affiliations:
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Le document en format XML
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National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo</wicri:regionArea><wicri:noRegion>64100 Teramo</wicri:noRegion></affiliation></author><author><name sortKey="Megdich, Aida" sort="Megdich, Aida" uniqKey="Megdich A" first="Aida" last="Megdich">Aida Megdich</name><affiliation wicri:level="4"><nlm:affiliation>Univérsité de Tunis El Manar, Institut de la Recherche Vétérinaire de Tunisie (IRVT), Laboratoire de virologie, 20 Rue Djebel Lakhdar, 1006 Tunis, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Univérsité de Tunis El Manar, Institut de la Recherche Vétérinaire de Tunisie (IRVT), Laboratoire de virologie, 20 Rue Djebel Lakhdar, 1006 Tunis</wicri:regionArea><placeName><settlement type="city">Tunis</settlement><region nuts="2">Gouvernorat de Tunis</region></placeName><orgName type="university">Université de Tunis - El Manar</orgName><placeName><settlement type="city">Tunis</settlement><region type="region" nuts="2">Gouvernorat de Tunis</region></placeName></affiliation></author><author><name sortKey="Portanti, Ottavio" sort="Portanti, Ottavio" uniqKey="Portanti O" first="Ottavio" last="Portanti">Ottavio Portanti</name><affiliation wicri:level="1"><nlm:affiliation>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo</wicri:regionArea><wicri:noRegion>64100 Teramo</wicri:noRegion></affiliation></author><author><name sortKey="Seliman, Imed Ben" sort="Seliman, Imed Ben" uniqKey="Seliman I" first="Imed Ben" last="Seliman">Imed Ben Seliman</name><affiliation wicri:level="1"><nlm:affiliation>CRDA, Medenine, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>CRDA, Medenine</wicri:regionArea><wicri:noRegion>Medenine</wicri:noRegion></affiliation></author><author><name sortKey="Spedicato, Massimo" sort="Spedicato, Massimo" uniqKey="Spedicato M" first="Massimo" last="Spedicato">Massimo Spedicato</name><affiliation wicri:level="1"><nlm:affiliation>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo</wicri:regionArea><wicri:noRegion>64100 Teramo</wicri:noRegion></affiliation></author><author><name sortKey="Pizzurro, Federica" sort="Pizzurro, Federica" uniqKey="Pizzurro F" first="Federica" last="Pizzurro">Federica Pizzurro</name><affiliation wicri:level="1"><nlm:affiliation>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo</wicri:regionArea><wicri:noRegion>64100 Teramo</wicri:noRegion></affiliation></author><author><name sortKey="Carmine, Irene" sort="Carmine, Irene" uniqKey="Carmine I" first="Irene" last="Carmine">Irene Carmine</name><affiliation wicri:level="1"><nlm:affiliation>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo</wicri:regionArea><wicri:noRegion>64100 Teramo</wicri:noRegion></affiliation></author><author><name sortKey="Teodori, Liana" sort="Teodori, Liana" uniqKey="Teodori L" first="Liana" last="Teodori">Liana Teodori</name><affiliation wicri:level="1"><nlm:affiliation>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo</wicri:regionArea><wicri:noRegion>64100 Teramo</wicri:noRegion></affiliation></author><author><name sortKey="Mahjoub, Mejdi" sort="Mahjoub, Mejdi" uniqKey="Mahjoub M" first="Mejdi" last="Mahjoub">Mejdi Mahjoub</name><affiliation wicri:level="1"><nlm:affiliation>CRDA, Gafsa, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>CRDA, Gafsa</wicri:regionArea><wicri:noRegion>Gafsa</wicri:noRegion></affiliation></author><author><name sortKey="Mangone, Iolanda" sort="Mangone, Iolanda" uniqKey="Mangone I" first="Iolanda" last="Mangone">Iolanda Mangone</name><affiliation wicri:level="1"><nlm:affiliation>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo</wicri:regionArea><wicri:noRegion>64100 Teramo</wicri:noRegion></affiliation></author><author><name sortKey="Leone, Alessandra" sort="Leone, Alessandra" uniqKey="Leone A" first="Alessandra" last="Leone">Alessandra Leone</name><affiliation wicri:level="1"><nlm:affiliation>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo</wicri:regionArea><wicri:noRegion>64100 Teramo</wicri:noRegion></affiliation></author><author><name sortKey="Hammami, Salah" sort="Hammami, Salah" uniqKey="Hammami S" first="Salah" last="Hammami">Salah Hammami</name><affiliation wicri:level="1"><nlm:affiliation>Ecole Nationale de Médecine Vétérinaire de Sidi Thabet, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Ecole Nationale de Médecine Vétérinaire de Sidi Thabet</wicri:regionArea></affiliation></author><author><name sortKey="Marcacci, Maurilia" sort="Marcacci, Maurilia" uniqKey="Marcacci M" first="Maurilia" last="Marcacci">Maurilia Marcacci</name><affiliation wicri:level="1"><nlm:affiliation>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo</wicri:regionArea><wicri:noRegion>64100 Teramo</wicri:noRegion></affiliation></author><author><name sortKey="Savini, Giovanni" sort="Savini, Giovanni" uniqKey="Savini G" first="Giovanni" last="Savini">Giovanni Savini</name><affiliation wicri:level="1"><nlm:affiliation>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo</wicri:regionArea><wicri:noRegion>64100 Teramo</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:29386141</idno><idno type="pmid">29386141</idno><idno type="doi">10.1016/j.meegid.2018.01.025</idno><idno type="wicri:Area/PubMed/Corpus">000377</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000377</idno><idno type="wicri:Area/PubMed/Curation">000376</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000376</idno><idno type="wicri:Area/PubMed/Checkpoint">000382</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000382</idno><idno type="wicri:Area/Main/Merge">000434</idno><idno type="wicri:Area/Main/Curation">000434</idno><idno type="wicri:Area/Main/Exploration">000434</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Analysis of bluetongue serotype 3 spread in Tunisia and discovery of a novel strain related to the bluetongue virus isolated from a commercial sheep pox vaccine.</title><author><name sortKey="Lorusso, Alessio" sort="Lorusso, Alessio" uniqKey="Lorusso A" first="Alessio" last="Lorusso">Alessio Lorusso</name><affiliation wicri:level="1"><nlm:affiliation>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo, Italy. Electronic address: a.lorusso@izs.it.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo</wicri:regionArea><wicri:noRegion>64100 Teramo</wicri:noRegion></affiliation></author><author><name sortKey="Sghaier, Soufien" sort="Sghaier, Soufien" uniqKey="Sghaier S" first="Soufien" last="Sghaier">Soufien Sghaier</name><affiliation wicri:level="4"><nlm:affiliation>Univérsité de Tunis El Manar, Institut de la Recherche Vétérinaire de Tunisie (IRVT), Laboratoire de virologie, 20 Rue Djebel Lakhdar, 1006 Tunis, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Univérsité de Tunis El Manar, Institut de la Recherche Vétérinaire de Tunisie (IRVT), Laboratoire de virologie, 20 Rue Djebel Lakhdar, 1006 Tunis</wicri:regionArea><placeName><settlement type="city">Tunis</settlement><region nuts="2">Gouvernorat de Tunis</region></placeName><orgName type="university">Université de Tunis - El Manar</orgName><placeName><settlement type="city">Tunis</settlement><region type="region" nuts="2">Gouvernorat de Tunis</region></placeName></affiliation></author><author><name sortKey="Di Domenico, Marco" sort="Di Domenico, Marco" uniqKey="Di Domenico M" first="Marco" last="Di Domenico">Marco Di Domenico</name><affiliation wicri:level="1"><nlm:affiliation>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo</wicri:regionArea><wicri:noRegion>64100 Teramo</wicri:noRegion></affiliation></author><author><name sortKey="Barbria, Mohamed Elias" sort="Barbria, Mohamed Elias" uniqKey="Barbria M" first="Mohamed Elias" last="Barbria">Mohamed Elias Barbria</name><affiliation wicri:level="1"><nlm:affiliation>CRDA-Commissariats Régionaux au Developpement Agricole, Menzel Bouzelfa, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>CRDA-Commissariats Régionaux au Developpement Agricole, Menzel Bouzelfa</wicri:regionArea><wicri:noRegion>Menzel Bouzelfa</wicri:noRegion></affiliation></author><author><name sortKey="Zaccaria, Guendalina" sort="Zaccaria, Guendalina" uniqKey="Zaccaria G" first="Guendalina" last="Zaccaria">Guendalina Zaccaria</name><affiliation wicri:level="1"><nlm:affiliation>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo</wicri:regionArea><wicri:noRegion>64100 Teramo</wicri:noRegion></affiliation></author><author><name sortKey="Megdich, Aida" sort="Megdich, Aida" uniqKey="Megdich A" first="Aida" last="Megdich">Aida Megdich</name><affiliation wicri:level="4"><nlm:affiliation>Univérsité de Tunis El Manar, Institut de la Recherche Vétérinaire de Tunisie (IRVT), Laboratoire de virologie, 20 Rue Djebel Lakhdar, 1006 Tunis, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Univérsité de Tunis El Manar, Institut de la Recherche Vétérinaire de Tunisie (IRVT), Laboratoire de virologie, 20 Rue Djebel Lakhdar, 1006 Tunis</wicri:regionArea><placeName><settlement type="city">Tunis</settlement><region nuts="2">Gouvernorat de Tunis</region></placeName><orgName type="university">Université de Tunis - El Manar</orgName><placeName><settlement type="city">Tunis</settlement><region type="region" nuts="2">Gouvernorat de Tunis</region></placeName></affiliation></author><author><name sortKey="Portanti, Ottavio" sort="Portanti, Ottavio" uniqKey="Portanti O" first="Ottavio" last="Portanti">Ottavio Portanti</name><affiliation wicri:level="1"><nlm:affiliation>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo</wicri:regionArea><wicri:noRegion>64100 Teramo</wicri:noRegion></affiliation></author><author><name sortKey="Seliman, Imed Ben" sort="Seliman, Imed Ben" uniqKey="Seliman I" first="Imed Ben" last="Seliman">Imed Ben Seliman</name><affiliation wicri:level="1"><nlm:affiliation>CRDA, Medenine, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>CRDA, Medenine</wicri:regionArea><wicri:noRegion>Medenine</wicri:noRegion></affiliation></author><author><name sortKey="Spedicato, Massimo" sort="Spedicato, Massimo" uniqKey="Spedicato M" first="Massimo" last="Spedicato">Massimo Spedicato</name><affiliation wicri:level="1"><nlm:affiliation>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo</wicri:regionArea><wicri:noRegion>64100 Teramo</wicri:noRegion></affiliation></author><author><name sortKey="Pizzurro, Federica" sort="Pizzurro, Federica" uniqKey="Pizzurro F" first="Federica" last="Pizzurro">Federica Pizzurro</name><affiliation wicri:level="1"><nlm:affiliation>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo</wicri:regionArea><wicri:noRegion>64100 Teramo</wicri:noRegion></affiliation></author><author><name sortKey="Carmine, Irene" sort="Carmine, Irene" uniqKey="Carmine I" first="Irene" last="Carmine">Irene Carmine</name><affiliation wicri:level="1"><nlm:affiliation>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo</wicri:regionArea><wicri:noRegion>64100 Teramo</wicri:noRegion></affiliation></author><author><name sortKey="Teodori, Liana" sort="Teodori, Liana" uniqKey="Teodori L" first="Liana" last="Teodori">Liana Teodori</name><affiliation wicri:level="1"><nlm:affiliation>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo</wicri:regionArea><wicri:noRegion>64100 Teramo</wicri:noRegion></affiliation></author><author><name sortKey="Mahjoub, Mejdi" sort="Mahjoub, Mejdi" uniqKey="Mahjoub M" first="Mejdi" last="Mahjoub">Mejdi Mahjoub</name><affiliation wicri:level="1"><nlm:affiliation>CRDA, Gafsa, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>CRDA, Gafsa</wicri:regionArea><wicri:noRegion>Gafsa</wicri:noRegion></affiliation></author><author><name sortKey="Mangone, Iolanda" sort="Mangone, Iolanda" uniqKey="Mangone I" first="Iolanda" last="Mangone">Iolanda Mangone</name><affiliation wicri:level="1"><nlm:affiliation>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo</wicri:regionArea><wicri:noRegion>64100 Teramo</wicri:noRegion></affiliation></author><author><name sortKey="Leone, Alessandra" sort="Leone, Alessandra" uniqKey="Leone A" first="Alessandra" last="Leone">Alessandra Leone</name><affiliation wicri:level="1"><nlm:affiliation>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo</wicri:regionArea><wicri:noRegion>64100 Teramo</wicri:noRegion></affiliation></author><author><name sortKey="Hammami, Salah" sort="Hammami, Salah" uniqKey="Hammami S" first="Salah" last="Hammami">Salah Hammami</name><affiliation wicri:level="1"><nlm:affiliation>Ecole Nationale de Médecine Vétérinaire de Sidi Thabet, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Ecole Nationale de Médecine Vétérinaire de Sidi Thabet</wicri:regionArea></affiliation></author><author><name sortKey="Marcacci, Maurilia" sort="Marcacci, Maurilia" uniqKey="Marcacci M" first="Maurilia" last="Marcacci">Maurilia Marcacci</name><affiliation wicri:level="1"><nlm:affiliation>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo</wicri:regionArea><wicri:noRegion>64100 Teramo</wicri:noRegion></affiliation></author><author><name sortKey="Savini, Giovanni" sort="Savini, Giovanni" uniqKey="Savini G" first="Giovanni" last="Savini">Giovanni Savini</name><affiliation wicri:level="1"><nlm:affiliation>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Teramo, Italy; National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo</wicri:regionArea><wicri:noRegion>64100 Teramo</wicri:noRegion></affiliation></author></analytic><series><title level="j">Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases</title><idno type="eISSN">1567-7257</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Bluetongue (virology)</term><term>Bluetongue virus (classification)</term><term>Bluetongue virus (genetics)</term><term>Female (MeSH)</term><term>Poxviridae Infections (prevention & control)</term><term>Poxviridae Infections (virology)</term><term>RNA, Viral (blood)</term><term>RNA, Viral (genetics)</term><term>Serogroup (MeSH)</term><term>Sheep (virology)</term><term>Sheep Diseases (prevention & control)</term><term>Sheep Diseases (virology)</term><term>Tunisia (MeSH)</term><term>Viral Vaccines (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (génétique)</term><term>ARN viral (sang)</term><term>Animaux (MeSH)</term><term>Femelle (MeSH)</term><term>Fièvre catarrhale du mouton (virologie)</term><term>Infections à Poxviridae (prévention et contrôle)</term><term>Infections à Poxviridae (virologie)</term><term>Maladies des ovins (prévention et contrôle)</term><term>Maladies des ovins (virologie)</term><term>Ovis (virologie)</term><term>Sérogroupe (MeSH)</term><term>Tunisie (MeSH)</term><term>Vaccins antiviraux (génétique)</term><term>Virus de la langue bleue (classification)</term><term>Virus de la langue bleue (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Viral Vaccines</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Tunisia</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Bluetongue virus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Bluetongue virus</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term><term>Vaccins antiviraux</term><term>Virus de la langue bleue</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Poxviridae Infections</term><term>Sheep Diseases</term></keywords><keywords scheme="MESH" qualifier="prévention et contrôle" xml:lang="fr"><term>Infections à Poxviridae</term><term>Maladies des ovins</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>ARN viral</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Fièvre catarrhale du mouton</term><term>Infections à Poxviridae</term><term>Maladies des ovins</term><term>Ovis</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Bluetongue</term><term>Poxviridae Infections</term><term>Sheep</term><term>Sheep Diseases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>Serogroup</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Femelle</term><term>Sérogroupe</term><term>Tunisie</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Tunisie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Bluetongue (BT), is one of the OIE-listed major diseases of ruminants. Following the official report of BT virus serotype 3 (BTV-3) in a sheep in Cap Bon (Tunisia), blood and serum samples of ruminants were collected from some areas of Tunisia to further investigate the presence of this virus in the country. A quantitative real time RT-PCR has been first developed for the detection and quantitation of BTV-3 RNA from field specimens. Out of 62 collected blood samples, 23 were shown to be positive for BTV-3 RNA. Isolation on cell cultures was also possible from six samples. Genome sequencing revealed the circulation of two unrelated western strains of BTV-3, one circulating in Cap Bon and neighboring areas, and the other circulating nearby the border with Libya. The presence of a putative novel BTV serotype (BTV-Y TUN2017) in sheep introduced from Libya to Tunisia, genomically related to the BTV strain contaminating a commercially-available sheep pox vaccine and to BTV-26, has been also demonstrated. This finding highlights the pressing need for a prompt production and release of a novel inactivated BTV-3 vaccine to be used in case of emergence or proactively in the areas of Southern Europe at major risk of BTV introduction. The assessment of a novel vaccine will certainly exalt the role and importance of surveillance activities and collaboration with Northern African countries.</div></front></TEI><affiliations><list><country><li>Italie</li><li>Tunisie</li></country><region><li>Gouvernorat de Tunis</li></region><settlement><li>Tunis</li></settlement><orgName><li>Université de Tunis - El Manar</li></orgName></list><tree><country name="Italie"><noRegion><name sortKey="Lorusso, Alessio" sort="Lorusso, Alessio" uniqKey="Lorusso A" first="Alessio" last="Lorusso">Alessio Lorusso</name></noRegion><name sortKey="Carmine, Irene" sort="Carmine, Irene" uniqKey="Carmine I" first="Irene" last="Carmine">Irene Carmine</name><name sortKey="Di Domenico, Marco" sort="Di Domenico, Marco" uniqKey="Di Domenico M" first="Marco" last="Di Domenico">Marco Di Domenico</name><name sortKey="Leone, Alessandra" sort="Leone, Alessandra" uniqKey="Leone A" first="Alessandra" last="Leone">Alessandra Leone</name><name sortKey="Mangone, Iolanda" sort="Mangone, Iolanda" uniqKey="Mangone I" first="Iolanda" last="Mangone">Iolanda Mangone</name><name sortKey="Marcacci, Maurilia" sort="Marcacci, Maurilia" uniqKey="Marcacci M" first="Maurilia" last="Marcacci">Maurilia Marcacci</name><name sortKey="Pizzurro, Federica" sort="Pizzurro, Federica" uniqKey="Pizzurro F" first="Federica" last="Pizzurro">Federica Pizzurro</name><name sortKey="Portanti, Ottavio" sort="Portanti, Ottavio" uniqKey="Portanti O" first="Ottavio" last="Portanti">Ottavio Portanti</name><name sortKey="Savini, Giovanni" sort="Savini, Giovanni" uniqKey="Savini G" first="Giovanni" last="Savini">Giovanni Savini</name><name sortKey="Spedicato, Massimo" sort="Spedicato, Massimo" uniqKey="Spedicato M" first="Massimo" last="Spedicato">Massimo Spedicato</name><name sortKey="Teodori, Liana" sort="Teodori, Liana" uniqKey="Teodori L" first="Liana" last="Teodori">Liana Teodori</name><name sortKey="Zaccaria, Guendalina" sort="Zaccaria, Guendalina" uniqKey="Zaccaria G" first="Guendalina" last="Zaccaria">Guendalina Zaccaria</name></country><country name="Tunisie"><region name="Gouvernorat de Tunis"><name sortKey="Sghaier, Soufien" sort="Sghaier, Soufien" uniqKey="Sghaier S" first="Soufien" last="Sghaier">Soufien Sghaier</name></region><name sortKey="Barbria, Mohamed Elias" sort="Barbria, Mohamed Elias" uniqKey="Barbria M" first="Mohamed Elias" last="Barbria">Mohamed Elias Barbria</name><name sortKey="Hammami, Salah" sort="Hammami, Salah" uniqKey="Hammami S" first="Salah" last="Hammami">Salah Hammami</name><name sortKey="Mahjoub, Mejdi" sort="Mahjoub, Mejdi" uniqKey="Mahjoub M" first="Mejdi" last="Mahjoub">Mejdi Mahjoub</name><name sortKey="Megdich, Aida" sort="Megdich, Aida" uniqKey="Megdich A" first="Aida" last="Megdich">Aida Megdich</name><name sortKey="Seliman, Imed Ben" sort="Seliman, Imed Ben" uniqKey="Seliman I" first="Imed Ben" last="Seliman">Imed Ben Seliman</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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